Abstract

WOUND HEALING POTENTIAL OF METHANOLIC EXTRACT AND ITS ACTIVE FRACTION OF LAWSONIA ALBA LAM LEAVES FORMULATED IN TO A TOPICAL GEL

Soumyasree Dutta, Ashok Kumar Pattnaik, Shila Elizabeth Besra*

Abstract

This study evaluated the wound healing potential leaves of Lawsonia alba Lam by excision and incision wound model. Besides this activity we also studied the acute inflammation by carrageenan model and analgesic study by using tail immersion method in mice. The methanol extract and its active fraction contained glycosides, flavonoids, tannins, coumarins and quinines and were relatively stable when stored at room temperature for three months. Solvent free semi solid extract of Lawsonia alba was incorporated into an aqueous gel and applied (5%W/W) on excision and incision wounds in Swiss albino mice. Excision wound model results showed that all treated animal groups (Standard colloidal silver gel and 5% test extract gel) exhibited significant increase in the percentage of wound contraction as compared to control group from 5th to 32nd days. In incision wound model treated with ELA showed significant increase in tensile strength as compared to control. Both the MLA and ELA were found to possess anti-inflammatory and analgesic activities at 1/10th of its LD50 dose of 100mg/kg and 50mg/kg i.p. respectively. MLA and ELA also enhanced peritoneal cell count and the number of macrophages in normal mice. It is plausible that the active fraction of MLA may be responsible for these activities of leaves of Lawsonia alba Lam.

Keywords: Wound healing, Lawsonia alba Lam. gel, excision and incision model, peritoneal macrophage.