Abstract

EXTRACTION, CHARACTERIZATION AND OPTIMIZATION OF PIGMENT PRODUCED FROM SERRATIA MARCESCENS

Dr. K. P. Renukadevi* and M. Vineeth

Abstract

The present study was carried out to extract pigment from Serratia marcescens. The bacterium Serratia marcescens was isolated from waste water sample and characterized both morphologically and biochemically. Further identification of the Serratia sp were done by 16rRNA sequencing and confirmed to be Serratia marcescens. The pigment was extracted from Serratia marcescens by HCl acid extraction method and the sample was purified for further analysis. Purification of the pigment was done by column chromatography and the pigments were separated from the impurities by silica gel plate using methanol, ethyl acetate, chloroform, water, in the ratio of 6:3:1:1. The pink color spot was observed under UV light which confirms the pigment as prodigiosin like compound.

Keywords: Serratia marcescens, Pigment, Optimization, Thin layer chromatography, Column Chromatography.