Abstract

STUDIES ON INFLAMMATORY MARKERS (IL-1, IL-4) IN BREAST CANCER DISEASE PROGRESSION

Safoora Habeeb,* Dr. Kaiser Jamil, Javeed Ahmed and Dr. M. Uma Devi

Abstract

Awareness and understanding of cancer disease will help in making correct decisions. The aim of the present studies involves identification and confirmation of role of interleukins as inflammatory markers in breast cancer disease progression. Cytokines play a very important role in nearly all aspects of inflammation and immunity. 'Interleukins' (ILs) are a group of cytokines with complex immunomodulatory functions. They also play an important role in immune cell differentiation and activation. ILs have pro- and anti-inflammatory effects. In the present work the patient‟s complete history, clinical symptoms and pathology of the disease were studied. Blood was drawn from 60 individuals (30 cases and 30 controls), DNA was isolated from the blood samples of cases and controls. The genes that code for IL-1β and IL-4(VNTR intron 3) were amplified by PCR using gene specific primers followed by RFLP, its product was then resolved on agarose gel electrophoresis and based on the RFLP and PCR patterns observed, the role of interleukins as inflammatory markers in breast cancer disease progression was identified and confirmed. Knowledge of this investigation will be useful for helping breast cancer patients to be diagnosed at early stages using IL markers. This will also help in identifying the correlation between existing risk factors and genetics.

Keywords: Breast cancer, Interleukins, risk factors.