Abstract

IN VITRO EFFECTS OF HYDROETHANOLIC EXTRACT FROM ROOTS BARK OF PARQUETINA NIGRESCENS (PERIPLOCACEAE) ON THE RABBIT DUODENUM CONTRACTILE ACTIVITY

André B. Konan*, Marie L.B. Ahui, Isidore A. Saraka, Augustin K. Amonkan, Léandre K. Kouakou, Mathieu N. Bléyéré, Jacques Y. Datté

Abstract

Hemorrhoidal disease is one of the most common diseases of the anal axis. The causes of this disease are numerous. This disease is manifested by disturbances of the intestinal transit, usually associated with atony of the handle and which contribute to a reduction in the force of peristaltic contractions of the large intestine. African pharmacopoeia in general and in particular the Ivorian pharmacopoeia contains various plants known anti-hemorrhoidal including Parquetina nigrescens (AfZ.) Bullock (Periplocaceae). Also, this study was carried to examine the in vitro effects of hydroethanolic extract from root bark of P. nigrescens on the contractile activity of rabbit duodenum. The isometric contractile force of the rabbit duodenum strips (RaDs) was recorded by using a strain gauge. P. nigrescens hydroethanlolic extract (EPan, 9.9×10-5 mg/ml – 0.2 mg/ml) showed myostimulant properties. These myostimulant effects were characterized by the increase of the amplitude and the basal tone of RaDs in normal Mac Ewen solution and by the development of phasic contractions in solution without calcium. In this Ca2+-free solution, the addition of EPan (9.9×10-3 mg/ml) elicited the development of phasic contraction in the presence of EGTA (0.1 mM). This result suggests that EPan could act on the double calcium flux (intracellular and extracellular). In conclusion, the hydroethanolic 70 % extract of P. nigrescens (EPan) had myostimulant effects (prostaglandin-like activity) on the rabbit isolated duodenum. The root bark of this herbal may increase peristalsis of intestinal muscle and thus increasing intestinal transit. Parquetina nigrescens has mysotimulant property thus supporting its folkloric usage to treat haemorrhoid.

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