Abstract

MOLECULAR CHARACTERIZATION OF CAPSULAR POLYSACCHARIDE & PUTATIVE SERIENE PROTEASE GENES OF KLEBSIELLA PNEUMONIAE ISOLATED FROM PUBLIC HOSPITALS IN BAGHADAD - IRAQ

Duaa Firas Rasheed* and Dr. Falah Salim Manhal

Abstract

Gram negative pathogens are an important cause of hospital acquired infections throughout the world. Klebsiella pneumoniae is an important medicals pathogens responsible for causing nosocomial infections. About 158 bacterial samples & specimens have been collected [urine and sputum]. A total of 50 bacterial isolated were identified as Klebsiella pneumoniae in bacteriology department of Baghadad teaching hospital. The specimens were collected depending on the standard methods from hospitalized patients suffered from varaities of nosocomial infections. The study was conducted at Baghdad teaching hospital (medical city) from the period of January 2015 until June 2015 in order to investigate the role of capsular polysaccharide and putative serine protease genes as virulence factors of Klebsiella pneumoniae in nosocomial infections. The bacterial isolates were identified depending on the golden characterization of morphology and biophysiology. Results of biochemical tests were confirmed by using the API 20E and VITEK2 system which has been done in Central Public Health Laboratory (CPHL). Klebsiella pneumoniae clinical isolates were tested for antimicrobial sensitivity. The multiplex Polymerase chain reaction (Multiplex PCR) and gel-agarose electrophoresis has been done in Uruk Lab For Molecular & Serological tests in order to investigate the prescence and detection of [K1,K2,K57 capsular polysaccharide genes] and [htrAgene (putative seriene protease)] using PCR specific primers. It was found that only five isolates (10%) were positive to K2 cluster where as [K1,K57] not detected by PCR. K2 capsular type was commonly found among submitted isolates. The htrA gene was detected in 48 (96%) isolates from hospitalized patients with nosocomial pneumonia, ventilator-associated pneumonia (VAP), and catheters of urinary tract infections UTIs patients in the Intensive Care Units ICUs. It has found that this gene played an important and vital role in the Klebsiella pneumoniae virulence. The multiplex PCR method provide a rapid mean of characterization and typing of this important agent of community-acquried and nosocomial infections.

Keywords: Klebsiella pneumonia, Baghadad, varaities of nosocomial, biophysiology.