Abstract

GENETIC ANALYSIS OF GIARDIA LAMBLIA ISOLATED SPECIMENS FROM HUMANS IN SOME REGIONS OF BAGHDAD

Ihsan M. AL-Saqur*, Bedir M. Abbas and Hadeel A. Majeed

Abstract

The prevalence of Giardia lamblia was surved among 375 patient with gastroenteritis in outpatients of Medical City Lab, Al-Noamman Hospital and Al-Khadimya Teaching Hospital. In this study the relationship of some factors (gender, age and the season) with the prevalence of this intestinal protozoal parasite had been evaluated. the current study was carried out during the period from the beginning of April 2014 till the end of March 2015. The result revealed that the total rate of G.lamblia infection was 18.13% according to three diagnostic methods, there was no significant relation (p≤0.01) between infectivity rate of this parasite and gender, while there was significant relation(p≤0.01) between infectivity rate and the age groups, the age group (1-9) years had the highest rate (54.41%), a significant differences(p≤0.01) recorded between the infectivity rate and the seasons, as, the higher infection rate found in summer season (57.35%). Concerning G. lamblia molecular study, from the 68 positive samples, specific amplification of Trios Phosphate Isomerase (tpi)gene was noted in 38 (55.88%), the results showed that the PCR product of (tpi) gene used in detection G. lamblia genotype (assemblage A) and (assemblage B), that the positive samples isolates at 576bp and 208bp respectively, at percentage 10.52% and 68.42% respectively, and there are 21.05% mixed of assemblage (A+B).

Keywords: Giardia lamblia, Baghdad city, some factors, PCR, tpi gene.