Abstract

NOVEL METHOD FOR CHROMATOGRAPHIC DETERMINATION OF SETRALINE AND DOXOFYLLINE IN FORMULATION BY USING RP-HPLC

Usha Kondla* and E. Esther Rani

Abstract

A simple, rapid, selective, sensitive, linear, precise and accurate NOVALMETHOD FOR CHOMATOGRAPHIC DETERMINATION OF SETRALLINE AND DOXOFYLLINE. Tablet dosage form separation of drug was achieved on a reversed phase symmetry C18 column (150mm x 4.6mm) phosphate buffer was pH 3.5 & the mobile phase was optimized with consist of Acetonitrile, phosphate buffer mixed in the ratio of 30:70% v/v. The solution w3as chromatographed at constant flow rate of 1.0 ml / min. The UV detection wavelength was 234nm & 10 μm of sample was injected. Te linearity ranges of SERTRALINE & DOXOFYLINE were found to be 0.58μm from 1.77μg/ml of SERTRALINE & DOXOFYLINE 0.27 & 0.82μg/ml. Linear regression coefficient was more than 99-100% the value RSD is ranged from 0.37-0.53% indicating accuracy & precision of the method. LOD & LOQ were found to be within limit. The was validated as per ICH guidelines for its sensitivity, linearity, accuracy & precision. The method was successfully employed for routine quality control analysis SERTRALINE & DOXOFYLINE in its pharmaceutical formulations.

Keywords: Novalmethod for Chomatographic Determination of Setralline and Doxofylline.