Abstract

SCREENING, CHARACTERIZATION, IDENTIFICATION AND APPLICATIONS OF BIOACTIVE METABOLITES FROM MARINE ACTINOMYCETES - STREPTOMYCES VARIABILIS

Dr. P. Arumugam*, Aarthi Priyanga N., Saraswathi K.a, Sindhu S., Baskaran P.

Abstract

The main aim of the study was to isolate marine actinomycetes which are antagonistically potent to produce the secondary metabolites which have various industrial applications such as antimicrobial, antioxidant (Total antioxidant activity, DPPH assay and Fe3+ reducing power assay) and anticancer activity. Primary and Secondary screening for antimicrobial activity was determined by Cross streak method and Agar well diffusion method respectively. In pursuit of this marine sediments were collected from various locations in Chennai and from which five different actinomycetes have been isolated. Among the five isolates (ABTRI 1-5) the strain ABTRI 1 was found to be antagonistically active and its bioactive compounds were produced using ISP-2 medium. Crude bioactive compounds were then extracted and the compounds present within the crude extract of Marine Streptomyces ABTRI 1 were identified by Gas chromatography-Mass spectrometry analysis, which revealed the presence of seven bioactive compounds. The anticancer activity of the secondary metabolite of actinomycetes was evaluated on MCF-7 cell line by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The in vitro cytotoxicity assay on MCF-7 revealed that the secondary metabolite had cytotoxic effect with IC50 292.4μg/ml.

Keywords: Actinomycetes, DPPH, Thin layer chromatography, 16srRNA sequencing and MTT assay.