Abstract

MOLECULAR ANALYSIS OF GENETIC FIDELITY OF STEVIA REBAUDIANA BERT. USING RAPD MARKERS

Humera Nazneen*, P. Veera Reddy and S. Karnakar Reddy

Abstract

RAPD markers were used to evaluate the genetic fidelity of Stevia rebaudiana Bert. For RAPD analysis, total genomic DNA was extracted from 20 mg fresh leaves of mother and randomly selected 20 OPA RAPD primers tested produced amplification products that were monomorphic bands. For each primer major bands were scored and the size of the amplification products ranged between 500 bp-2.5 kb. A total of 92 bands were scored from PCR amplification of genomic DNA from four Samples (number of plantlets analyzed multiplied by number of bands with all primers). No polymorphism was detected after amplification by PCR among regenerated plants grown on different hormones. All the RAPD profile from all plants were monomorphic and comparable to other plants, confirming the genetic stability among micropropagated plants and mother plant. Those results could be very useful to study and manage the genetic resources of this important medicinal plant.

Keywords: KEYWORDS: Stevia rebaudiana , Asteraceae, Genetic Fidelity, RAPD, genetic similarity.