Abstract

EFFECT OF INHIBITORS ON CRUDE ALPHA-AMYLASE PRODUCED BY BREVIBACILLUS BORSTELENSIS R1 ISOLATED FROM COASTAL AREA OF BAY OF BENGAL, VISAKHAPATNAM

K. Suribabu* and K. P. J Hemalatha

Abstract

Alpha amylase have many applications in Bakery industry, Detaining in Laundry industry, Automation Dishwashing (Restaurants), Alcohol dual fermentation, Textile industry, Glucose Industry, Chocolate Syrup industry, Building product industry, Poultry feed Industry, Unmalted cereal liquefaction industry, Manufacture of maltose, Manufacture of high fructose containing syrups, Manufacture of high molecular weight branched dextrins etc. The control was the activity of the crude without inhibitor under standard conditions. All the inhibitors decreased the enzyme activity when compared with the control. The following were the activities of the crude enzyme in the presence of 0.3M AgNO3 (410±6 U/ml), 0.1M HgCl2 (383±3 U/ml), 0.1M ZnCl2 (370±6 U/ml), 0.1M CuSO4 (347±7 U/ml), 0.1M EDTA (327±7 U/ml) and 0.1M L-Glutamic acid (243±3 U/ml).

Keywords: Brevibacillus borstelensis R1, ?-amylase, AgNO3, HgCl2, EDTA, CuSO4, Lglutamic acid, ZnCl2.