Abstract

IN VITRO ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF ESSENTIAL OIL FROM THE SEEDS OF NEOLITSEA PALLENS (D. DON) MOM & HARA

Mohammad S. Javed,*, Pawan kumar, Sanjay Kumar, Rishendra Kumar, Shikha

Abstract

The essential oil from the seeds of Neolitsea pallens (D. Don) Moms & Hara growing in the Himalayan region is evaluated for its antioxidant activity using test for phenolic, flavanoid content and ferric reducing/antioxidant power assay (FRAP) and antibacterial activity by well diffusion method. The oil has shown 356.1 ± 0.003 mg/ml gallic acid equivalent per 100mg of the oil as total phenolic content and 355 ± 0.014 mg/ml quercetin equivalent per 100 mg of the oil as total flavonoid content. The oil exhibited marked antioxidant activity. The VEAC values were found to be 0.454g/L and 0.538g/L for the essential oil and ascorbic acid respectively. The ZOI for the test bacterial strains at a concentration of 500μg/ml were Gram negative bacteria Pseudomonas aeruginosa424 (7mm), Escherichia coli443 (4mm), Aeromonas hydrophila646 (4mm) and Gram positive bacteria Bacillus subtilis441 (4mm) and Staphylococcus aurieus737 (6mm).

Keywords: Neolitsea pallens, essential oil, antioxidant, antibacterial activity, infections.