Abstract

ISOLATION AND CHARACTERIZATION OF BERBERINE HCl AND CYTOTOXIC EFFECT ON THE CELL LINE OF BREAST CANCER

Jaspreet Kaur*, Dinesh Kumar Yadav, Swati Sharma, Mohsin Hasan, Pradeep Kumar Tyagi and Mahasweta Roy Singh

Abstract

In this research Berberine HCl was isolated from roots of Berberis aristata by ethanol using soxhlet apparatus. Identification of berberine was done by TLC and compound was determined by m.p., UV and FTIR Spectra. This study was planned to assess the cytotoxicity effect of berberine HCl on breast cancer cell line and the activity was carried out by using MCF-7 cell line. HPTLC profiling of the compound confirmed about the presence of Berberine and standard drug was compared with berberine. FT-IR was performed and compared with standard as well as isolated berberine. UV spectra and melting point was performed for isolated berberine. The cytotoxicity of standard and extracted berberine HCl was carried out by MTT assay and the results of extracted berberine were compared with standard berberine. It was found on the basis of UV, m.p., HPTLC and FT-IR that isolated compound is berberine HCl. FT-IR of standard and isolated berberine HCl were compared and found to be similar spectra. The % cytotoxicity of standard and extracted berberine at concentration of 12μM was found to be 82.19 % and 72.56 %. In cell viability assay, % cell viability of standard and extract Berberine HCl at concentration of 12μM was found to be 15.78% and 28.16%. The Berberine HCl was isolated and showed significant effect on MCF-7 cell line. In future prospect, in vivo study in animals models need to be performed to confirm the anticancer effect of Berberine HCl.

Keywords: Berberis aristata, cytotoxicity, Berberine HCl, Breast cancer.