Abstract

EVALUATION OF ESSENTIAL PHYTOCONSTITUENTS OF AEGLE MARMELOS USING LEAF EXTRACTS

A. U. Manjula* and Prema Sampath Kumar

Abstract

Medicinal plants are incredible source of bioactive constituents. Identification and elucidation of phytoconstituents leads to the discovery of drugs and to attain better approach to various ailments. Aegle marmelos (L.) Correa (Rutaceae) is a traditionally used medicinal plant known for siddha and ayurvedic formulations. The objective of this study was to identify and examine the bioactive chemical compounds and green synthesis of silver nanoparticles of dried leaves powder of A.marmelos subjected to solvents such as benzene, chloroform, methanol, ethanol and water (Aqueous). The phytochemical screening revealed the presence of essential therapeutic components such as alkaloids, flavonoids, terpenoids, tannin, glycosides, phenolic compounds, etc. The methanol and aqueous leaf extracts showed maximum active phytochemical compounds compared to the other solvents. TLC (Thin layer chromatography) profiling of leaves provides a clear sketch about the presence of potential phytoconstituents in the extract. The Ag+ ion reduction for silver nanoparticles synthesis was done using 1 mM silver nitrate (AgNO3) solution. The reduction was recorded at 418 and 422nm using UV-Visible spectrophotometer and SEM imaging confirmed the presence of cluster of spherical nanoparticle with the size of 20nm using ZEISS EVO 40 EP Electron microscope. Thus, the current research may be considered for selection of solvent for an ideal bioactivity studies and pharmacological research and also to obtain novel drugs using A.marmelos leaves.

Keywords: Aegle marmelos, Phytochemical, TLC, Silver nanoparticles, Characterization.