Abstract

ORAL DOSING OF METHYL PREDNISOLONE IN ADULT ZEBRAFISH AND EVALUATION OF HEPATO-PROTECTIVE ACTIVITY OF SUCCINATE DEHYDROGENASE AND ANTIOXIDANT ACTIVITY OF GSX AND GST

Renuka Jyothi S.*, Ashok D., Dr. B.P. Harini, Amitha R., Vibha Shenoy, Manjushree H. K.

Abstract

Methyl Prednisolone like most adrenocorticol steroids typically used for its anti-inflammatory effects. However, glucocorticoids have a wide range of effects, including changes to metabolism and immune responses. Common uses induces arthritis and short term treatment of bronchial inflammation or acute bronchitis due to various respiratory diseases. This study aims at use of Methyl Prednisolone and its effects on various organs and tissues of adult zebrafish and its related antioxidant enzymes like Glutathione Peroxide (GPx) and Glutathione- S-transperase and Succinate dehydrogenase enzyme. The LC 50 for Methyl Prednisolone was determined as 50ppm. As the concentration of drug dose was increased the activity of various enzymes was seen to increase exponentially. The activity of GPx was highest in muscles at both 20ppm and 10ppm followed by liver and gill had significant increase against control fishes. Similarly, the activity of GST and succinate dehydrogenase was highest in liver at both 20ppm and 10ppm followed by gill and muscle which had a significant increase against untreated zebrafish. The observation in this research reflects that all the enzymes activities were significantly enhanced in all the tissues (Gill, Muscle, liver) when compared to Methyl prednisolone unexposed ones. This obtained result could be due detoxification mechanism exhibited by the Danio rerio on exposure to Methyl Prednisolone.

Keywords: Methyl Prednisolone, Danio rerio, Glutathione Peroxide (GSx), Glutathione- S-transferase, Succinate dehydrogenase.