Abstract

TRICLOSAN INDUCED OXIDATIVE STRESS IN EPIDIDYMAL SPERM OF GOAT, CAPRA HIRCUS L. (1758) IN VITRO

C.V. Priyatha and K.C. Chitra*

Abstract

Triclosan is a widely used antimicrobial agent in pharmaceuticals and personal care products. The present study was undertaken to evaluate the influence of triclosan on the induction of oxidative stress in goat epididymal sperm in vitro. Sperm collected from mature fertile domestic male goat (Capra hircus L.) weighing 16 ± 2 Kg were incubated with triclosan in Ringer’s phosphate solution (RPS medium). Experiments were carried out in three groups, such as Group I: Single duration at different concentrations (50, 100, 200 and 300 μM/L for 5 h), Group II and III: Two different single concentrations (200 and 300 μM/L, respectively) at different durations (1, 2, 3, 4 and 5 h). Assay of antioxidant enzymes as superoxide dismutase, catalase and glutathione reductase were done in all groups. In addition, the levels of hydrogen peroxide generation and lipid peroxidation were also determined in all groups. In Group I, when epididymal sperm incubated for 5 h with triclosan, at 200 and 300 μM concentrations significantly decreased the activities of antioxidant enzymes and increased hydrogen peroxide generation and lipid peroxidation. In Group II, significant reduction in antioxidant enzymes with concomitant increase in lipid peroxidation were observed only after 4 h of incubation of sperm with triclosan. However, in Group III, the activities of antioxidant enzymes decreased significantly immediately after 1 h of exposure to triclosan with time-dependent significant increase in the levels of hydrogen peroxide and lipid peroxidation. The present results suggest that triclosan induced oxidative stress in epididymal sperm of goat in vitro.

Keywords: Triclosan, epididymal sperm, oxidative stress, antioxidant enzymes, goat.