Abstract

IDENTIFICATION OF BIOACTIVE COMPOUNDS IN LOESENERIELLA ARNOTTIANA WIGHT ROOT BY GC-MS ANALYSIS

*Prajna P.S., Rama Bhat P. and Sunil Kumar K. N.

Abstract

Loeseneriella arnottiana is commonly used in the folklore medicine for treating diabetes mellitus. Preliminary phytochemical analysis of root extract revealed the presence of tannins, flavonoids, saponins, glycosides, steroids, terpenoids, and alkaloids. N- hexane extract of L. arnottiana root was subjected to Gas Chromatography coupled with Mass Spectroscopy (GC-MS) for the identification of components thereon. GC-MS data indicates the presence of forty low polar components. Out of the 13 identified constituents, beta-amyrin (14.92%), squalene (9.97%), alpha-amryin (9.14%), benzenamine (1.51%), nonadecane (1.28%), 1-methyl-4-(1-methylethyl)-3-[1- methyl-1-(4-methylpentyl)-5-methylheptyl]cyclohexene (1.05%), octadecane (1.04%) were the major constituents. The remaining six constituents 11- Octadecenoic acid, methyl ester (0.91%), hexanedioic acid (0.89%), eicosane (0.55%), hexadecanoic acid, methyl ester (0.54%), 7,9-Di-tert-butyl-1-oxaspiro(4,5)deca-6,9-diene- 2,8-dione (0.38%), methyl stearate (0.37%) were found in trace amounts. Some of these constituents are known to possessanti-inflammatory, antidiabetic, antioxidant, anticancer and antimicrobial properties and hence L. arnottiana can be used as an active therapeutic ingredient.

Keywords: Loeseneriella arnottiana, GC-MS analysis, n-hexane extract, amyrins, antioxidant, antidiabetic.