Abstract

MARKER BASED STANDARDIZATION OF FORMULATIONS CONTAINING ASHWAGANDHA USING WITHAFERIN A BY HPLC

Dr Pratima A. Tatke* , Dr. Supriya S. Jirge and Dr. Satishchandra Y. Gabhe

Abstract

A high performance liquid chromatography (HPLC) method for the determination of Withaferin A in Withania somnifera formulations was developed. The developed and validated method was successfully applied to analyze the content of Withaferin A in different marketed formulations containing Ashwagandha. The method comprised of a simple isocratic system with reserve phase (RP)-18 column, acetonitrile and water as mobile phase and Withaferin A was detailed at 215 nm. The optimized mobile phase enabled the efficient separation of various components present in formulations within a 10 min analysis, with a retention time of 6.3 minutes for Withaferin A. The method was validated as per International conference of harmonization (ICH) guidelines with a view to demonstrate its selectivity, linearity, precision, accuracy and robustness. The linearity range was 100-1000 ng mL−1and the limit of detection (LOD) and limit of quantification (LOQ) were found to be 30 and 90 ng respectively. The accuracy assay performed revealed a recovery of 96.43-106.57 % from the various marketed formulations analyzed. The inter-day and intra-day reproducibilities were found to have an R.S.D. less than 0.1 %, indicating very good precision.

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