Abstract

OXIDATIVE SPECTROPHOTOMETRIC DETERMINATION OF DRUGS & PHARMACEUTICALS USING NBS AS OXIDANT & RHODAMINE-B DYE AS ANALYTICAL REAGENT

Rajitha Balusani*, Sayaji Rao, G. Sunitha3 and J. Ramchandar

Abstract

Objective: Simple, sensitive, precise and accurate method for quantitative determination of drugs viz., Alfuzosin (ALF), Atrovastatin Calcium (ATV) Ciprofloxacin (CIP) and Citalopram(CTP) have been developed. Method: This method depends upon the oxidation of the drugs by a known excess N-Bromosuccinimide (NBS) in Hydrochloric acid medium and subsequent determination of unreacted NBS by using Rhodamine-B dye. Results: This proposed method was applied for the determination of the quantity of the drug present in commercial tablets with no interference from the excipients. This method has been validated in terms of LOD, LOQ, precision accuracy, % RSD, robustness and ruggedness. Factors affecting the absorbance viz., concentration of HCl and time of reaction are optimized. The effect of excipients has also been studied and found to have no effect. The calibration curves are found useful for determination of pure drug and can be applied to pharmaceuticals in bulk drug and pharmaceutical industries.

Keywords: Objective: Simple, sensitive, precise and accurate method for quantitative determination of drugs viz., Alfuzosin (ALF), Atrovastatin Calcium (ATV) Ciprofloxacin (CIP) and Citalopram(CTP) have been developed. Method: This method depends upon the oxidation