Abstract

DETECTION CTX-M TYPES EXTENDED SPECTRUM β - LACTAMASES (ESBLs) IN ESCHERICHIA COLI ISOLATION FROM PREGNANT URINARY TRACT INFECTIONS (UTIs) IN BASRA, PROVINCE, IRAQ

Saad Shakir Mahdi*

Abstract

Urinary tract infections (UTIs) who are cause by E. coli an important etiologic agent of morbidity in the presently extended spectrum β- lactamases (ESBLs). Aim of present study was detection the types of CTX-M that spreading in the case of urinary tract infection between pregnant women. Current study were included the collection one hundred samples of the urine from pregnant women from, eighty samples given positive cultivation results, Gram-negative distributed after diagnosed using conventional techniques and conformed by using API 20E system to 37(46.25%) E.coli, 25(31.25%) Klebsiella spp., 12(15%) Pseudomonas spp. And 6(7.5%) Proteus spp. Results of detected ESBLs production were showed from 37(46.25%) E.coli isolates the 15(40.54%) ESBLs producing isolates, while 22(59.46%) non- ESBLs producing isolates. The second part of this study the amino acid sequencing data results for 15 plasmid DNA that identified (11) plasmid DNA in isolates (No3,7,11,25, No28,34,37,45,49 and No55,66) similarity the plasmid DNA have accession number in genbank (AJW2218.1, AEZ49563.1 and AMM70777.1) respectively as blaCTX-M-1 gene (100%), while (4) plasmid DNA in isolates (No30,72 and No 12,77) similarity the plasmid DNA have accession number in genbank (AGE61862.1 and ACU44517.1) respectively as blaCTX-M-15 gene (100%).

Keywords: CTX-M, ESBLs, E.coli, UTI.