Abstract

THROMBOLYTIC EFFECT OF THREE NSAIDS: IN VITRO AND IN SILICO APPROACH.

Abul Hasanat, Mohammad Shah Hafez Kabir*, Nurul Absar,
Mohammad Nazmul Islam, Mohammed Sohel Chowdhury, Imran Bin Habib, Mohammad Nazmul Hoque Jony, Md. Rakibul Hasan, Md. Nazmul Huda,
Mohammad Sahif Azad, Tanvir Ahmad Chowdhury

Abstract

The present study aims to investigate the thrombolytic effect of some NSAIDs by in vitro clot lysis method and in silico molecular docking used to identify whether these drugs interact with the responsible protein (tissue-type plasminogen activator). In vitro clot lysis model was used to observe the thrombolytic effect of paracetamol, aspirin and diclofenac sodium drugs, where they exhibited 42.59 ± 2.40%, 45.90 ± 2.67% and 33.43 ± 3.20% clot lysis, respectively. Reference drug streptokinase exhibited 78.70±0.92% clot lysis. A wide range of docking score found during molecular docking by CPI server. Paracetamol, aspirin and diclofenac sodium drugs showed the docking score -5.6, -6.0 and -1.3, respectively. Aspirin possessed highest clot lysis effect and also showed best docking score among the NSAIDs, where Diclofenac sodium exhibited the opposite. In both in vitro and in silico method, the drugs followed, aspirin > paracetamol > diclofenac sodium order for effect. Further in vivo investigation need to identify the thrombolytic effect of these NSAIDs and also require making out the mechanism of them as thrombolytic agents.

Keywords: NSAIDs, PASS prediction, Molecular docking, paracetamol, aspirin, diclofenac sodium.