Abstract

TOTAL PHENOL, TOTAL FLAVONOID CONTENT AND ANTIOXIDANT POTENTIAL OF METHANOL EXTRACT OF BOEHMERIA PLATYPHYLLA D DON LEAVES.

Mohammad Nazmul Islam*, Mohammad Shah Hafez Kabir, Syed Md. Abdul Kader, Mahmudul Hasan, Ebrahim Khan Samrat, Imran Bin Habib, Mohammad Nazmul Hoque Jony, Mohammed Sohel Chowdhury, Abul Hasanat, Md. Masudur Rahman

Abstract

Background: Boehmeria platyphylla leaves are deliberated as worthy traditional medicine. To give a scientific basis for traditional usage of this medicinal plant, the leaf extract were appreciated for its antioxidant activity. Methods: In this study, in vitro antioxidant activities of the leaf extract of B. platyphylla were determined by total antioxidant, DPPH, Reducing power capacity, total phenolic content and total flavonoid content. Result: The extract showed a dose dependent radical scavenging effect in DPPH assay. IC50 for free radicals achieved by the extract is 144.72 μg/ml. The extract showed significant reducing power activity as compared to ascorbic acid and proportionally increased with the increasing concentration of the extract. Increase in absorbance of the reaction mixture indicates the increase in the reducing power of the extract. Phenol content was 18.44 ± 0.45 mg gallic acid/g and flavonoid content was 21.12 ± 0.23 mg quercetin/g. Conclusion: Our current results emerged that B. platyphylla act as an antioxidant agent due to its free radical scavenging and cytoprotective activity. So, the plant may be further pursued to find out for its pharmacological active natural products.

Keywords: Boehmeria platyphylla, phenol, flavonoid, antioxidant, DPPH.