Abstract

DETECTION TOXOPLASMA GONDII IN BLOOD SAMPLES OF WOMEN ABORTIFACIENTS AND THEIR SPOUSES IN THE BASRA PROVINCE BY USING TG HSP70GENE AND B1 GENE

Sabeeh H. AL-Mayah, Shayma'a. J. Raisan* and Fayha N. AL-Malky

Abstract

Toxoplasmosis is one of the most important of parasitic infections in humans and animals, this parasite caused abortion and death of fetus in humans and mammalian animals. This study aimed to use Toxoplasma gondii70 kDa Heat Shock Protein (Tg HSP 70) and B1gene for the diagnosis of toxoplasmosis in blood of human by using the technique of polymerase chain reaction assay (PCR). Study were examined (300) blood samples from married patients and (50) blood samples as control for detection the presence of Toxoplasma gondii DNA in blood of patients by using Tg hsp70 and B1 gene. The 242 (80.7%) blood samples was given positive results for B1and (Tg hsp70) compared With control samples who are show a negative results for this test. Also observed in the current study, the infection in female show at ratio rate 136(90.7%)Compared with their spouses show at ratio rate106(70.7%) this results show no significant different between of theme and highest infection rate by toxoplasmosis show in the 31-35 age group at ratio (90.5%) compared with other age group.

Keywords: Toxoplasmosis, gene Tg hsp 70, B1 gene, polymerase chain reaction.