Abstract

SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF ALOGLIPTIN AND GLIBENCLAMIDE INSYNTHETIC MIXTURE

*Rana Anjali V. and Patel Paresh U.

Abstract

The present manuscript describe simple, sensitive, rapid, accurate, precise and economical spectrophotometric method based on simultaneous equation method for the simultaneous determination of Alogliptin and Glibenclamide in synthetic mixture. The method is based on the simultaneous equations for analysis of both the drugs using methanol as solvent. Alogliptin has absorbance maxima at 275.7 nm and Glibenclamide has absorbance maxima at 229.7 nm in methanol. The linearity were obtained in the concentration range of 1- 14 μg/ml for Alogliptin and 2-16μg/ml for Glibenclamide. The concentrations of the drugs were determined by using simultaneous equations method. The mean recovery was 101.09 ± 0.57 and 100.12 ± 0.97 for Alogliptin and Glibenclamide respectively. The method was found to be simple, sensitive, accurate and precise and was applicable for the simultaneous determination of Alogliptin and Glibenclamide in synthetic mixture. The results of analysis have been validated statistically and by recovery studies.

Keywords: Alogliptin, Glibenclamide, Simultaneous equations, Validation.