Abstract

ANTIBIOTIC RESISTANCE OF STAPHYLOCOCCUS AUREUS AND COAGULASE-NEGATIVE STAPHYLOCOCCI (CNS) ISOLATED FROM BOVINE MASTITIS IN THE REGION OF NORTH KARNATAKA, INDIA

Sadashiv S. O and B. B. Kaliwal*

Abstract

The present investigation was carried out to determine the species and antimicrobial susceptibilities of the Staphylococci isolated from clinical and subclinical Bovine mastitis milk. The samples were collected from North Karnataka, India (09 districts) from March 2012 to August 2012. A total of 392 Milk samples suffering from mastitis were screened and a total of 375 staphylococcus species were recovered. The molecular characterization confirmed that the isolates belong to Staphylococcus aureus, Staphylococcus sciuri, and Staphylococcus saprophyticus. The isolates were subjected to the antibiotic resistance screening. The overall antibiotic resistance test showed that the isolated Staphylococcus species were resistant to Methicillin (100%) followed by Penicillin G (95.97%), Cefpodoxime (88.85%), Oxacillin (87.61), Cefaclor (73.61%), Cefixime (72.91%), Ampicillin (69.73%), Ceftriaxone (61.94%), Streptomycin (36.77%), Amoxyclav (35.16%), Erythromycin (27.96%), Amikacin (24.99%), Norfloxacin (17.4%), Gentamicin (14.91%), Azithromycin (12.4%), Tetracycline (9.62%), Chloramphenicol (8.78%), Ciprofloxacin (5.14%), Ofloxacin (3.76%) and all the Staphylococcus strains were susceptible to Vancomycin. The present study demonstrated the presence of alarming level of resistance of frequently and commonly used antimicrobial agents to the isolated bacteria. Therefore, an examination of the antibiotic resistance profiles of the isolates may be recommended earlier to the use of antibiotics in both treatment and prevention of the disease.

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