Abstract

IDENTIFICATION AND QUANTIFICATION OF LUPEOL IN FORMULATION CONTAINING FICUS SPECIES USING HIGHPERFORMANCE THIN LAYER CHROMATOGRAPHY

Valsamma Wilson*, Dr. Sugandha S. Shetye, Kawaljit Kaur, Satish N. Ambare

Abstract

There is a growing demand for biocompatible natural products and formulations for the treatment of various new era diseases like Cancer, Parkinson‟s, Alzheimer‟s, and Diabetes. Plants have many phytochemicals with different bioactivities like antioxidant, antiinflammatory, and anticancer activities. In the present study, we report the identification and quantification of Lupeol, an anticancer agent, from an Ayurvedic formulation „Nalpamaradi choorna‟ and its four Ficus species constituents using High-Performance Thin Layer Chromatography (HPTLC). The chromatographic separation of the methanol extract of the samples was done on TLC alumina plates coated with silica gel 60 F254 as the stationary phase with Toluene: Ethyl acetate: Formic acid (5:5:0.5 v/v/v) as the mobile phase. The plates were scanned at reflectance absorbance mode at 540 nm after derivatizing with Anisaldehyde- Sulphuric acid reagent. The system was found to give compact spots with a Rf value of 0.72±0.02. The method was found to be linear in a range of 50-250 ng with a correlation coefficient r = 0.9982. The results showed the presence of 0.021%, 0.024%, 0.019%, 0.0.027% and 0.029% of Lupeol in F. benghalensis, F. racemosa, F.religiosa, F. microcarpa and the formulation respectively.

Keywords: HPTLC, Nalpamaradi Choorna, Ficus species, Lupeol, Anticancer activity.