Abstract

QUALITATIVE ANALYSIS AND QUANTITATIVE DETERMINATION OF “CURCUMIN” IN A SIDDHA HERBO-MINERAL FORMULATION USING HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC)

Shanmugapriya P.* and Murugesan M.

Abstract

Objective: To perform qualitative and quantitative estimation of „Curcumin‟ in a Siddha herbo-mineral formulation – „SanthaSanthrodayamMathirai (SSM)‟ using High-Performance Thin Layer Chromatography (HPTLC). Method: Methanolic extracts of three samples of SSM (A, B, C) prepared using the traditional procedures described in Siddha literature. High Performance Thin Layer Chromatography method was performed for quantification of curcumin in all the three samples by employing chloroform: methanol 9.5:0.5 (v/v) as a mobile phase. Results: Curcumin was identified at UV- 366 nm using the Retention factor (Rf) values in the three samples of SSM with reference to standard curcumin. The percentage of curcumin present in sample A, B and C is 0.6589, 0.6884 and 0.7104 respectively. Conclusion: Qualitative analysis and quantitative estimation of Curcumin content in SanthaSanthrodayaMathirai (SSM) was successfully done using High-Performance Thin Layer Chromatography (HPTLC). These results suggest that quantification of Curcumin in SSM formulation can be helpful in quality control and the curcumin fingerprints obtained by HPTLC can be helpful in SSM standardization.

Keywords: Curcumin, High-Performance Thin Layer Chromatography, Santha Santhrodaya Mathirai.