Abstract

HERBAL FORMULA MTR-88 ENHANCES ADIPOGENESIS IN 3T3-L1 CELLS AND INSULIN SENSITIVITY IN OBESE MICE

Yoshida Hiroki* and Sang-G Jung

Abstract

According to previous studies, the inability of fat tissues to properly expand or respond to insulin, may generate metabolic dysfunctions. The purpose of this study is to investigate adipocytes and obese mice to understand if MTR-88, composed of Anemarrhena asphodeloides and Myristica fragrans extracts, can control the development of fat cells. We studied whether this extract generates migration, lipid accumulation, and differentiation in 3T3-L1 cells and verified this through Oil Red O staining and the quantitative PCR. The blood glucose level for insulin, adiponectin and AktpS (473) phosphorylation were analyzed in the animal experiments. Anemarrhena asphodeloides and Myristica fragrans extracts promoted the expression of PPARγ, C/EBP-alpha and ADIPOQ and improved the phosphorylation reaction of AktpS473 in obese mice. In addition, we confirmed that MCP-1 levels were reduced and aiponectin in blood increased. We reveal that this natural extract can have a positive metabolic effect on fat cells and white adipose tissues.

Keywords: MTR-88; 3T3-L1; Adipogenesis; obese; diabetes mellitus.