Abstract

INVESTIGATIONS ON MOLECULAR MECHANISM INVOLVED IN NEUROPROTECTIVE EFFECT OF VITAMIN D AGAINST SODIUM AZIDE INDUCED ALZHEIMER'S DISEASE IN RATS.

Neha Atwal*, Chanpreet Singh Bindra and Upendra K. Jain

Abstract

The present study is undertaken to investigate the beneficial role of Vitamin D in sodium azide (SAZ) induced Alzheimer's disease (AD) in rats. This study also aimed to explore the potential role of PPAR-γ in Vitamin D mediated neuroprotection against i.p SAZ. administered rats. SAZ (12.5 mg/kg, i.p. for 5 days and 10 mg/kg, i.p. for next 9 days) was administered to rats, their learning and memory assessment is done by Morris Water Maze (MWM) test. After that various biochemical parameters were performed i.e acetylcholinesterase (AChE) activity, nitrite/ nitrate activity, myeloperoxidase (MPO) activity, thiobarbituric acid reactive substances (TBARS) level, glutathione (GSH) level. The study also showed that i.p. SAZ impaired the learning and memory and also increased the levels of brain AChE, nitrite/ nitrate, MPO and TBARS and decrease in GSH level. Pre-treatment with bisphenol-A-diglycidyl ether (BADGE), a selective PPAR-γ antagonist, significantly abolished the beneficial effect of Vitamin D in i.p. SAZ treated animals. This investigation lead to results, which document a potential role of PPAR-γ in Vitamin D mediated neuroprotection against i.p. SAZ induced Alzheimer's disease.

Keywords: Morris water maze, PPAR-?, myeloperoxidase activity, inflammation, oxidative stress, acetylcholinesterase activity, Alzheimer's disease.