Abstract

FT-IR ANALYSIS AND IN VITRO ANTI-INFLAMMATORY ACTIVITY OF LEAF EXTRACT OF MICHELIA CHAMPACA (L.)

T. Ananthi* and R. Kalaiselvi

Abstract

The present study is to investigate the Phytochemical, FT-IR and invitro anti- inflammatory activity of ethanol leaf extract of Michelia champaca. The preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, phenols, saponins, tannins, steroids, triterpenoids, coumarins, glycoside, and reducing sugar. FT-IR spectrum confirmed the presence of (O-H str), (C=C str), (C-H str), (C=C str), (C-H bond), (C-N str), (C-O str), (C C-H: C-H bond) groups. The anti-inflammatory activity was evaluated using albumin denaturation assay and membrane stabilization at different concentrations (200, 400, 600, 800, 1000 μg/ml). The in vitro antiinflammatory activity of the extract was concentration dependent, with the increasing concentration, the activity is also increased. Aspirin was used as standard drug. The percentage of inhibition was compared with those of standard drugs. The maximum percentage inhibition was observed at a dose of 1000μg/ml. From the results, it is concluded that secondary metabolites present in the leaf extract responsible for the anti-inflammatory activity.

Keywords: Michelia champaca, anti-inflammatory activity, albumin denaturation, membrane stabilization, aspirin.