Abstract

ANTIBACTERIAL ACTIVITY OF EXTRACT OBTAINED FROM CATHARNTHUS ROSUES AGAINST DIFFERENT PATHOGENIC BACTERIA

Sheeraz Ahmad Wagay*, Ab Rashid Wani, Ghulam Mohammad Jan, Tawseef Ahmad Bhat and Surayakant

Abstract

Catharanthus roseus family Apocynaceae is highly reputed medicinal tree commonly known as the Sadabahar. Various tissues of Catharanthus roseus have demonstrated positive effects on heart diseases (root bark), dysentery and inflammation (leaves), diabetes (leaf extract), and ulcers. In the present study the antibacterial activity of the methanol, chloroform, ethanol, & aqueous extract from the leaves of Catharanthus roseus was studied using disc diffusion method & agar well method against pathogenic bacterial strains of Achromobacter, Bacillus, Klebsiella, CoNS (Coagulase negative staphylococcus), Enterobacter, Enterococci, Pseudomonas, Proteus, Staphylococcus aureus, E. coli. Best result showed in all extract against Pseudomonas, Enterococci, S. aureus and Bacillus in a well diffusion method but S. aureus showed highest zone of inhibition in methanol extract. In paper disc method ethanol extract showed best result against Pseudomona sp.

Keywords: Catharanthus roseus; Pathogenic bacteria; Agar well diffusion method; Disc diffusion method; Inhibition zone.