Abstract

HOST-PARASITE RELATIONSHIPS IN TISSUE CULTURE OF SAFFLOWER AND FUSARIUM OXYSPORUM F.SP. CARTHAMI

Ramesh N. Dhawale* Roshan N. Jadhav and Dr. Kamlakar K. Kamble

Abstract

Among the different diseases affecting safflower, wilt disease caused by Fusarium oxysporum f.sp. carthami observed in severe form, this disease spread widely and causes yield losses up to 80%. Microscopy was developing reliable in vitro assays may provide a basis for the use of interactions in dual cultures in vitro to evaluate the pathogenicity of fungi and susceptibility of host plant genotype. The pathogenicity of fungi and susceptibility of host plant genotypes along with the degree of threat posed by fungi was evaluated. This may also enable the selection on embryonic level that is more resistant to the pathogen, which is particularly important for fungi known for their ability of epiphytic occurrence. The margin of the leaf lamina were cultured in the MS medium supplemented with different concentration 0.5 to 2.5 mg/L of 2,4-D there was formation of abundantly growing callus, in concentration of 2,4-D (2.0 mg/L) callus on entire surface of leaf. After 3-4 weeks few green nodules were observed on the surface of white transparent nodulated callus. Changes due to Fusarium oxysporum f.sp. carthami fungus infection to callus give reliable results which can be used for selection of disease resistant genotype. The present investigation was conducted for standardization of in vitro callus induction protocol conducted for the variety GMU and to study effect of Fusarium carthami on callus of safflower at cellular level by microscopic study.

Keywords: Safflower, Fusarium carthami, host-parasite, pathogenecity, 2, 4-D, GMU.