Abstract

IN-VITRO FREE RADICAL SCAVENGING ACTIVITY STUDIES OF EXTRACTS AND ISOLATED COMPOUNDS OF EUGENIA JAMBOLANA LAM. SEEDS

Sasikala M.*, Karpakavalli M., Ranjithkumar D., Prakash G., Vanathi P., Mohan S.

Abstract

Background: Eugenia jambolana seeds of Myrtaceae family are substantial ayurvedic medicine in treating diabetes. It is also narrated to have anti-inflammatory, hypoglycemic, antiulcerative, hypolipidemic, antiviral, ameliorative and antioxidant activities due to flavonoids, tannins, triterpenoids, saponins and glycosides. Objective: Various successive Soxhlet extractives [petroleum ether (PEE), CHCl3 (CE), ethyl acetate (EAE) and methanol (ME)] and isolated compounds [IC1 and IC2] of E. jambolana seeds were inflicted to free radical scavenging activities employing DPPH and H2O2 radicals procedure which could be the main cause of degenerative diseases. Materials and Methods: All the extracts and isolated compounds were established to predicate good antioxidant activity against DPPH and H2O2 radicals as compared to standard Ascorbic acid. PEE and CE showed very less antioxidant activity when compared to standard, EAE, ME, IC1 and IC2. Standard, PEE, CE, EAE, ME, IC1 and IC2 offered utmost DPPH radical scavenging activity of 88.49, 17.40, 22.59, 61.08, 72.12, 54.55 and 66.62 μg/ml at concentration of 500 μg/ml and for H2O2 scavenging, ultimate activity of 82.09, 24.25, 27.23, 67.61, 78.85, 60.53 and 71.36 μg/ml at concentration of 50 μg/ml. The antioxidant activity was extreme in ME and IC2. Results: The IC50 of Standard, EAE, ME, IC1 and IC2 for DPPH radical and H2O2 radical was pioneered 265, 360, 295, 380 and 220 μg/ml and 26, 33, 27,37 and 27 μg/ml respectively. Conclusion: The separation and identification of flavonoids, tannins, glycosides and triterpenoid saponins present in seeds of E. jambolana can help the researchers to detect new molecules as natural antioxidants.

Keywords: Antioxidant, DPPH, Eugenia jambolana, H2O2, IC50, Isolated compound.