Abstract

OPTIMISATION OF PARAMETERS FOR ENHANCED TANNASE PRODUCTION FROM A NOVEL BACTERIAL PRODUCER

Sarang Sheela*, Vichare Smita and Vora Dipak

Abstract

Tannase (E.C. 3.1.1.20) is an inducible hydrolysable enzyme of great value in various industries. It hydrolyses the ester and depside linkages in hydrolysable tannins to release glucose and gallic acid. Tannase has gained importance in the food, chemical, beverage and pharmaceutical industry as a means of providing cost effective gallic acid. Hence different strategies are required to increase the production of tannase without compromising economic considerations. In this paper, we have reported the isolation and characterisation of a novel bacterial tannase producer. Furthermore, the media components were optimised to achieve high tannase yields using environmentally friendly methods. The effect of nitrogen and carbon sources as well as the effect of tannic acid concentration were studied with a view to obtain high tannase yields. In addition, physical conditions such as the effect of temperatures, pH, aeration and agitation were also investigated. These manipulations have resulted in an overall 1.4 fold increase in tannase production. These studies suggest that the tannase producer under consideration has the potential of being exploited commercially.

Keywords: Tannase, green technology, isolation, media optimisation, high yields.