Abstract

ANTIOXIDANTS ACTIVITY OF EUCALYPTUS CAMALDULENSIS AGAINST ACETAMINOPHEN WHICH CAUSES OXIDATIVE STRESS IN MALES RATS

*Wid Abass Mohammed and Jawad Khadim Faris

Abstract

The present study has been designed to evaluate the beneficial effects of Eucalyptus camaldulensis extract upon acetaminophen-induced renal toxicity, The study has been conducted on adult male rats at the department of physiology, College of Veterinary Medicine, Al-Qasim Green University during the period extended from November, 2015 to February, 2016. Forty mature male Wistar rats (aged 90 days and weighted 190±10 g) were divided in to four randomly equal groups, the first served as negative control received only distilled water, the second as positive control received with Acetaminophen (2.5 g/kg b. w.) as a single dose. third and fourth groups received Acetaminophen (2.5 g/kg b. w.) as a single dose then treated after 2 hours with EUE (150,200 mg /kg b.w.) respectively orally through stomach tube during 42 days. males were anaesthetized (by injection IM of 0.3ml ketamine + 0.1 ml of xylazine /kg b. w. ip), blood samples were obtained from heart in non-heparinized tubes, Blood serum samples were separated for assessment of MDA, SOD, CAT, GSH concentration. Under our experimental conditions, acetaminophen poisoning resulted in an oxidative stress evidenced by statistically significant losses in the activities of catalase (CAT), superoxide-dismutase (SOD), Glutathione (GSH) and increase in lipids peroxidation(MDA) level in Blood serum of acetaminophen-treated group compared with the control group, and after the gavage Eucalyptus camaldulensis extract alcoholic has been results demonstrated statistically significant increase activity in catalase (CAT), superoxide-dismutase (SOD), Glutathione (GSH) and significant decrease in lipids peroxidation(MDA) level in Blood serum of AE150.AE200 group compared with the Acetaminophen-treated group.

Keywords: Acetaminophen, Eucalyptus, Males rats, Superoxide dismutase (SOD), Glutathione (GSH), Catalase (CAT), and Malondialdehyde (MDA).