Abstract

PREVALENCE OF GENES ENCODING ENTEROTOXINS AND EXFOLIATIVE TOXINS AMONG METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS CLINICAL ISOLATES IN IRAQ

Ali J. Saleem*, Nizar E. Nasser and Munim R. Ali

Abstract

In current study, 140 clinical isolates of Staphylococcus aureus were collected from different hospitals in Baghdad and Diyala. 113 of them were diagnosed as Methicillin-resistant Staphylococcus aureus (MRSA) by two different methods were used; phenotypic method was done by chromogenic MRSA agar and genotypic method was done by Polymerase Chain Reaction (PCR) technique to detection mecA gene. Toxins genes were investigated using seven specific primers, five of them for the detection of enterotoxins genes that encoding for sea, seb, sec, sed and see which showed the prevalence in percentage (24.78%, 0%, 38.82%, 19.47% and 11.5%) respectively, and two primers for the detection of exfoliative toxin genes that encoding for eta and etb which showed the prevalence in percentage (5.31% and 7.96%) respectively.

Keywords: Staphylococcus aureus, sea, seb, sec, sed and see.