Abstract

EFFECT OF AQUEOUS EXTRACT OF BLACK SEED(NIGELLA SATIVA) AND AQUEOUS EXTRACT OF TRIGONELLAFOENUM IN SOMEHISTOLOGICAL AND BIOCHEMICAL CHANGES TOTRICHOMONASVAGINALIS

Reham N. Al-Timimi*, Dr. Shatha Khudair and Dr. Saad A. Al-Jashaami

Abstract

Trichomonasvaginalis is a unicellular protozoan flagellate parasite, that has only the trophozoites stage. It has no cystic stage in its life cycle. This study was designed to investigate the in vivo inhibitory activity of Nigella sativa and Trigonellafoenum aqueous extract on the growth of Trichomonasvaginalis in comparison to metronidazole, and determine effect of different concentrations of Nigella sativa and Trigonellafoenum seeds extract of pathological physiological, histological and Biochemical changes on mice infected with Trichomonasvaginalis. This study was carried out during the period from Noveber, 2015 to April, 2016. Vaginal swabs and urine samples were collected from AL-habebia hospital ad Ibn Al-balady hospital and Al-ulweiya hospitals for women and childbirth in Baghdad. Collect samples of women who suffer from the symptoms of the disease vaginitis, Itching and burning. swab specimen was immediately placed in 5mL of Diamond modified medium. 80 of female mice in age two month and weight (25-35 gm) given 105 trophozoites/ml in the vagina. During 6-day swabs taken from the vagina of mice and examined under light microscope in order to know the number of trophozoite and the impact of aqueous extract on the growth of parasites and compared with the control positive and with the treatment of metronidazole. The best results of the highest inhibition on the sixth day were for Flagyl, as well as treatment with Nigella sativa 10 mg/ml and the mixture of N.sativa and T. foenum 5 mg/ml followed from other transactions and significantly less than 0.01 compare with positive control. The Biochemical test of Glucose, Creatinin and Alkaline-phosphatase show Glucose level increase in Flagyl group (227.25 ± 8.9 b mg/dl) and its level increase in Control +ve (241.3 ± 1.3 a mg/dl), N. sativa 5 mg/ml (193.54 ± 2.94 d mg/dl), N. sativa 10 mg/ml (211.66 ± 32.9 c mg/dl), T. foenum 5 mg/m (181.66 ± 24.6 d mg/dl), T. foenum 10 mg/ml (192.91 ± 1.4 d mg/dl), compared with Control –ve group (171.27 ± 0.9 e mg/dl). the difference was significant between all treatments except between the Control –ve and mixture N.sativa and T.foenum 5mg/ml(172.21 ± 27.2 e mg/dl) difference was non-significant (P˂0.01). Level of creatinin in serum decrease in the rate in Flagyl group (0.14 ± 0.05 cmg/dl), N. sativa 5 mg/ml (0.145 ± 0.05 c mg/dl), N. sativa 10 mg/ml (0.135 ± 0.05 c mg/dl), T. foenum 5 mg/m (mg/dl), mixture N.sativa and T. foenum 5mg/ml (0.155 ± 0.09 c mg/dl) ,and Control + ve (0.155 ± 0.05 c mg/dl) compared with Control –ve group (171.27 ± 0.9 e mg/dl). the difference was significant between all treatments except between the Control –ve and T. foenum 10 mg/ml was non-significant (P˂0.01). ALkaline-phosphatase level decrease in Flagyl group (119.82 ± 10.91 c U/l), N. sativa 5 mg/ml (125.77 ± 9.6 b U/l), N. sativa 10 mg/ml (125.19 ± 16.11 b U/l), T.foenum 5 mg/m (85.06 ± 1.71 d U/l), T. foenum 10 mg/ml (56.27 ± 2.5 e U/l), mixture N.sativa and T.foenum 5mg/ml (113.11 ± 17.9 c U/l), and in Control + ve (0.23 ± 0.08 U/l) compared with Control –ve group (186.67 ± 2.11 a U/l). the difference was significant between all treatments (P˂0.01). Results showed diverse histopathological changes in the uterus and vagina of infected animals with Trichomonasvaginalis included inflammatory cellular infiltration, secretory activity and hyperplasia of epithelia cell.

Keywords: Trichomonasvaginalis Trigonellafoenum histopathological cell.