Abstract

ISOLATION OF THERMO ALKALINE PROTEASE PRODUCING BACTERIA AND EVALUATION OF AGRO-WASTE FOR PROTEASE PRODUCTION

Asma Farheen and Lingappa K.*

Abstract

In the present study, a potential protease producing isolate designated ASKL-09 was obtained from the garden soil by serially dilution. Based on morphological, microscopic, biochemical and 16S rRNA sequencing the isolate was identified as Bacillus sonorensis. The optimum pH and temperature was pH 9 and 50ËšC respectively at 30 h. Carbon source (glucose at 1.5 %) and nitrogen source (beef extract 0.5%) and metal ion (magnesium sulphate at 0.05%) showed highest protease production. Bacillus sonorensis ASKL-09 produces protease at high pH and temperature suggesting it could useful for industrial applications.

Keywords: Alkaline protease; submerged fermentation; agro-wastes; Bacillus sonorensis.