Abstract

IN VITRO MICROPROPAGATION OF DENDROCALAMUS STRICTUS (SOLID BAMBOO)

Dr. Bhanwar Lal Jat*, Dr. Rashmi Panwar, Dr. Dilip Gena, Mudasir Ahmad Mir, Rajveer Singh Rawat

Abstract

Multiples shoots were induced from in vitro seedlings of Dendrocalamus strictus on Murashige and Shoog‟s medium supplemented with B.A and kinetin. About 15-25 shoots were obtained within 20-25 days from nodal explants of seedling in the primary culture. The seedling derived cultures were separated into groups of 5- 7 and transferred to fresh subculture medium. Rooting of the shoots was achieved under in vitro and ex vitro conditions. 85 -90% of rooting was achieved by the ex vitro method using IBA. A rapid and highly effective method for Micropropagation of Dendrocalamus strictus was established. Seeds of Dendrocalamus strictus were inculcated on MS medium containing 4.5gm/lit agar and 3% commercial sugar and different combinations of 6- Benzyladenine (BA) with Kinetin (KN). 100% of seeds were germinated on the medium without any growth hormone compared to the medium containing BA (0.5-1.0mg/lit). Nodal segments were proved to be the best region comparison to the shoot tips having high rate of shoot induction and more number of shoots multiplication. Maximum rate of multiplication were observed in the medium containing BA (1.0-3.0mg/lit).

Keywords: GH/GR-(Growth Hormone/ Growth Regulators), DW- (Distilled Water), AC- (Activated Charcoal), CW/CM-(Coconut Water/ Coconut Milk).