Abstract

NEW QUANTITATIVE ESTIMATION OF ACETAZOLAMIDE BULK SAMPLE USING HYDROTROPIC SOLUBILIZING AGENTS

Mahavir Chhajed¹*, Atika Chhajed², Anil Shrivastava³, Saurabh Gupta⁴, Shaily Mogra⁵

¹SAFE Institute of Pharmacy, Gram Kanadiya, Indore. (M.P.)

²Devi Ahilya College of Pharmacy, Chhawani, Indore. (M.P.)

³Anand College of Pharmacy, Mathura Road, Agra. (U.P.)

⁴J.S.S. College of Pharmacy, Rockland’s Ootacamund, Ooty. (T.N.)

⁵Mordern Institute of Pharmaceutical Sciences, Indore. (M.P.)

Abstract

In the present investigation, hydrotropic solubilization technique has been employed to solubilize the poorly water soluble antidiuretic drug, acetazolamide for its spectrophotometric analysis. For the same work N,N’-dimethyl urea solution (by 7.5 M), and sodium acetate solution (5.5 M) were used as hydrotropic agent. There was more than 2 fold and 1.8 fold enhancement in aqueous solubility of acetazolamide in 7.5 M N,N’-dimethyl urea solution and 5.5 M sodium acetate solution respectively; compared to the solubility in distilled water. The hydrotropic agent did not interfere in the analysis. The hydrotrop is economic and pollution free. The proposed method is new, simple, accurate and reproducible. Statistical data proved the accuracy, reproducibility and precision of the proposed method.

Keywords: Hydrotropy, Acetazolamide, N,N’-dimethyl urea, sodium acetate,Spectrophotometry.