Abstract

ANALGESIC ACTIVITIES OF METHANOL EXTRACT OF PHYLLANTHUS SIKKIMENSIS MUELL. ARG. LEAVES

Md. Abdullah Al Masum, Mohammad Shah Hafez Kabir*, Mohammed Shoibe, Mahmudul Hasan, Nishan Chakrabarty, Md. Jakiul Islam, Monisha Basak, Md. Nazmul Hasan, Md. Hosne Mobarak Chowdhury, Mohammed Abu Sayeed

Abstract

Objectives: The present study was to investigate analgesic action of the methanol extract of Phyllanthus sikkimensis. Methods: The animals were submitted to acetic acid-induced writhing test and Formalin induced licking test to assess analgesic activities, respectively. Two doses 400 and 200 mg/kg were administered for testing. Results: The methanol extract of P. sikkimensis (MEPS) at both doses, exhibited a significant (p < 0.05 - < 0.01) dose-dependent analgesic activity in acetic acid writhing test and Formalin test. In acetic acid-induced writhing test, oral administration of MEPS (200 and 400 mg/kg) also decreased the writhing significantly while compared to control. The dose 400 mg/kg showed maximum percentage of pain inhibition 45.63% and 65.73% for respectively. Diclofenac sodium (10 mg/kg) was used as reference analgesic drugs. MEPS (200 and 400 mg/kg) significantly suppressed the licking activity in both phase of the formalin-induced pain in mice in a dose dependant manner. The reference analgesic drug Diclofenac sodium (10 mg/kg) also significantly inhibited the licking activity against both phases of formalin induced pain. Conclusions: The leaves extract has potential analgesic activity. The present study supports the use of P. sikkimensis in different pain states.

Keywords: P. sikkimensis, analgesic, Acetic acid writhing test, Formalin.