Abstract

NEW CYTOTOXIC ANTHRAQUINONE GLUCOSIDE FROM ATRIPLEX NUMMULARIA L.

Abd El Raheim M. Donia*, Asmaa M. Radwan, Ahmed M. Zaghloul

Abstract

Objective: the present study aims to investigate the phytochemical content of Atriplex nummularia and evaluate their cytotoxic activity. Methods: The ethyl acetate extract of A.nummularia was subjected to fractionation using Silica gel chromatographic column and ethyl acetate methanol eluting system. The structure of the isolated compound was elucidated using 1HNMR, 13CNMR, COSY, HSQC, HMBC, DEPT, FT-IR and LC-MS. Cytotoxic activity of this compound was evaluated against Caco-2, HCT, HepG-2 and MCF-7 Cell lines. Results: A new cytotoxic anthraquinoneglucoside was isolated for the first time from Atriplex nummularia L. and named 1- hydroxy-3-hydroxymethyl-6--D-O-glucopyranosyl-anthracene-9,10- dione. It showed significant cytotoxic activity against different cell lines. The highest activity appeared against HCT followed by MCF-7.

Keywords: Atriplex nummularia, Anthraquinone, Cytotoxic activity, Caco-2, HepG-2, MCF-7, HCT cell lines.