Abstract

QUANTIFICATION OF PHARMACOLOGICALLY ACTIVE MARKER LUPEOL AND PHYTOL FROM LEAF AND STEM OF PERGULARIA DAEMIA FORSK BY HPTLC METHOD

Sutar N. G.*, Pal S. C. and Ritu Gilhotra

Abstract

TLC densitometry method for quantification of Lupeol and Phytol using HPTLC is developed. This is the first report of quantification of these two biologically active compounds as Lupeol and Phytol using HPTLC from this plant. Quantification of Lupeol was carried out from the petroleum ether extract by using a solvent system of Toluene: Methanol (99:1) and for Phytol Chloroform: Methanol (99:1). The Rf value of Lupeol and Phytol are 0.67 and 0.22 respectively. The linearity ranges of Lupeol and Phytol were (100 to 600ng) with correlation coefficient (r- values) of 0.996 and 0.993 respectively. The amount of Lupeol and Phytol was 1.65% w/w and 0.0007%v/w in leaf and 1.75%w/w and 0.023 %v/w in stem respectively. Quantification of Lupeol and phytol were showed good separation and resolution from other constituent of the extract. The main advantages are its simplicity, selectivity and accuracy. This method can also be employed for estimation of these compounds in other herbal preparation and may be useful for standardization purposes.

Keywords: Pergularia daemia Forsk, HPTLC, Lupeol and Phytol.