Abstract

EFFECT OF QUERCETIN ON SOME OF ANTIOXIDANT ENZYMES IN WISTAR RATS EXPOSURE TO THE OXIDATIVE STRESS BY LEAD ACETATE

Muntasser Alawi Awad AL Awadi*, Wed Abbas Mohammed and Hayder Abdul Kadem ALzamely

Abstract

The present study was aimed to investigate the beneficial effect of quercetin extracted from onions to improvement of some antioxidant enzymes in adult males rats exposed to the oxidative stress by lead acetate. Forty males Wistar rats about 6 months old with average weight175±10gm were divided randomly into the four equal groups and treated for 60 days as follows: The first group C was given drinking water only as control group. The second group (T1) was given quercetin (300 mg/kg/ B.w) orally, the third group (T2) given lead acetate (10 mg/kg/ B.w) orally and the fourth group was given lead acetate (10mg/kg/B.w) orally for 30 day then given quercetin (300mg/kg/B.W) for 30 day. In the end of experiment all animals were sacrificed and samples of blood were collected from the abdominal vein by using 5 ml disposable syringe then putting in gel and clot activator tube and left at room temperature until clotted, then putting it in centrifuge at (5000 rpm) for (5 min), the serum separated from the tube and stored in -20 until using for assessment of antioxidant enzymes. The results showed there was significant increase (p≤0.05) in malondialdehyde (MDA) level in T2 group compared with other groups. Also there was a significant decrease in MDA level in T1 compared with other groups .While there were no significant difference in MDA level in T3 compared with control group. Also there was a significant increase (p≤0.05) in superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT) concentration in T1group compared with other groups and there was a significant decrease in T2 group compared with other groups. Also there were no significant difference in SOD and CAT in T3 group compared with control group.

Keywords: Quercetin, antioxidant enzymes, oxidative stress, lead acetate.