Abstract

SPUTUM SELECTION, SMEAR PREPARATION, STAINING AND MICROSCOPY TECHNIQUES FOR AFB (REVIEW)

Manoj Verma, Kirti Jain and Sangeeta Mahale*

Abstract

Tuberculosis is a highly infectious diseases with about world’s population including 40% Indian estimated to be infected. An estimated 8.6 million cases developed TB and 1.3 million died from the disease. In diagnosis of lower respiratory tract infection sputum smear microscopy is the most commonly used technique. This review gives the details used in TB diagnosis smear preparation by direct and concentration method, Smear staining by Z N, Florescent microscopy techniques.

Keywords: TB diagnosis, Sputum, AFB.