Abstract

IN VITRO ANTIOXIDANT SCREENING OF FUCOIDON EXTRACTED FROM KAPPAPHYCUS ALVAREZII (RED SEAWEED)

P. Rajasekar* and K. Jeyaprakash

Abstract

Objective: The present study was carried out to evaluate the antioxdidant potential and toxicity effect of polysaccharide-fucoidon from the red seaweed Kappaphycus alvarezii. Fucoidon a polysaccharide contain fucose and sulphate as major compounds. Interestingly, various studies reported that the presence of sulphated content of the sample play a significant role in pharmacological activities. In this study fucoidon extract from Kappaphycus alvarezii were tested for different phytoconstituents and the in vitro antioxidant activity was studied by using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power activity, hydrogen peroxide scavenging activity, superoxide scavenging activity and nitric oxide scavenging activity. Results: The highest free radical scavenging activity of DPPH assay, super oxide radical scavenging activity, reducing power assay, hydroxyl radicals assay and nitric oxide radical scavenging activity, was found to be 70.91±4.96 μg/ml, 83.21±5.82μg/ml, 0.78±5.46μg/ml, 67.50±4.72μg/ml, 76.19±5.33μg/ml, at highest concentration of methanol extract of 80 μg/ml respectively in this study. Conclusion: The present study concluded the in vitro antioxidant activity of fucoidon extracted from Kappaphycus alvarezii which might be due to the presence of the antioxidant compounds.Hence ,fucoidon have the scope of being used us natural antioxidants in treating many human diseases.

Keywords: Fucoidon, Antioxidant, Kappaphycus alvarezii, Scavenging activity.