Abstract

DEVELOPMENT AND VALIDATION OF LC-MS/MS METHOD FOR THE SIMULTANEOUS DETERMINATION OF QUINONES IN TECTONA GRANDIS LINN

Shailendra Rane*, Manish Hate, Prashant Hande, Ajitkumar BS and Ajit Datar

Abstract

A simple, precise and accurate LC-MS/MS method has been developed for simultaneous determination of four quinones viz. 2- methylanthraquinone, 5-hydroxy-1,4-naphthoquinone, 2- (hydroxymethyl) anthraquinone and emodin in leaves powder of Tectona grandis linn. MRM (Multiple Reaction Monitoring) transitions 223.10>223.05, 174.00>174.20, 236.20>236.05 and 268.90>269.20 were optimized on Shimadzu triple quadrupole mass spectrometer instrument (Model: LCMS-8040) for quantification of 2- methylanthraquinone, 5-hydroxy-1,4-naphthoquinone, 2-(hydroxymethyl) anthraquinone and emodin respectively. Chromatographic method was developed on a Shimadzu shimpack-XR C18 column (75mm x 3.0mm x 2.2 μ) by gradient program. The proposed method was validated for linearity, accuracy, precision, recovery, limit of detection and limit of quantitation. The validated LC-MS/MS method can be used for a routine quality control analysis and simultaneous quantitation of quinones viz. 2-methylanthraquinone, 5-hydroxy- 1,4-naphthoquinone, 2-(hydroxymethyl) anthraquinone and emodin in Tectona grandis linn.

Keywords: LC-MS/MS, quinones, 2-methylanthraquinone, 5-hydroxy-1,4- naphthoquinone, 2-(hydroxymethyl) anthraquinone, emodin