Abstract

THE PROTECTIVE EFFECTS OF PHYLLANTHUS EMBLICA IN CYCLOPHOSPHAMIDE INDUCED GENOTOXICITY IN MICE

Keshava Rao K. and Rudrama Devi K.*

Abstract

In the present investigation the antimutagenic effects of Phyllanthus fruit extract (PFE) has been evaluated against cyclophosphamide induced genotoxicity in bone marrow cells of mice. The administration of Phyllanthus fruit extract at various doses i.e. 170, 340 and 680 mg /kg. When treated individually did not induce chromosomal aberrations in somatic cells of mice in 24hrs. A single Intraperitoneal administration of 50mg/kg of cyclophosphamide induced significant increase in the percentage of micronuclei in bone marrow cells of mice. However after co administration of three doses of PFE extract there was a dose dependent decrease in the % of micronuclei was observed. When animals were administered with Phyllanthus Fruit Extract PFE 170, 340 & 680 mg/kg/bw orally for two weeks and on sixteenth day CP (50 mg/kg/bw) was given intraperitonially as a single dose. For each experimental group control, animals were maintained. Two days after the administration of the last dose, the animals were sacrificed and air dried metaphase preparations were made and processed for identification of chromosomal aberrations in somatic cells of mice. In animals treated with single dose of CP, an increase was observed when compared with the values of control group. But when animals primed with PFE + CP group, there was a decrease in the frequency of chromosomal aberrations. Thus the results clearly indicated the protective role of PFE on cyclophosphamide induced genotoxic damage in somatic cells of mice.

Keywords: Cyclophosphamide, Phyllanthus bone marrow cells.