Abstract

INTRACELLULAR AND EXTRACELLULAR BIOSYNTHESIS OF SILVER NANOPARTICLES BY EXTREMOPHILIC BACTERIA

Dr. Rachana Singh and Vipul Verma

Abstract

The development of reliable processes for the synthesis of silver nanomaterials is an important aspect of nanotechnology today. There is enormous interest in developing green synthesis procedures for production of silver nanoparticles by using biomimetic approaches. There are few reports in literature on the biosynthesis of silver nanoparticles using microorganisms such as fungi. In our research focus has been given to the production of silver nanoparticles by an efficient, eco friendly viable process for the synthesis of silver nanoparticles using three halophilic and alkalihpilic bacterial strains Ra3, CD3 and CD13, isolated from different sources. A significant result of this study is that all the three bacterial strain are synthesizing silver nanoparticle intra and extracellularly. The shape and size of silver nanoparticle were characterized by scanning electron microscopy and UV- visible spectroscopy. The silver nanoparticle was effectively produced by all the three bacterial isolates. The method of extraction of intracellular and intracellular nanoparticles was inexpensive, simple and effective in large scale with no need to complex instruments. The bacteria work as a bio-nanofactory which continued to grow after synthesis of silver nanoparticles. This new approach of using non pathogenic bacterial strain for the successful synthesis of nanosized silvers could be easily scaled up which establishes its commercial validity.

Keywords: Silver nano particle, biosynthesis, extracellular, intracellular, microbial production.