Abstract

EFFECT OF AMLODIPINE BESYLATE FOR WOUND HEALING IN DIABETIC RATS

*Madhuri Grover, Dr J. J. Bhelonde and Pankhuri Raheja

Abstract

Objective: Amlodipine Besylate is a long acting Calcium Channel Blocker (CCB), used in the treatment of Hypertension worldwide. The present study aims to evaluate the wound healing potential of Amlodipine Besylate in diabetic Wistar Hannover (WH) rats. Materials and Methods: All the selected rats were divided into 5 groups: Non-diabetic control (group I), diabetic control (group II), diabetic treated with Amlodipine Besylate 2.5 mg/Kg (group III), 5 mg/Kg (group IV) and 10 mg/Kg (group V) respectively. Rats belonging to group I and II were given the vehicle (Methyl Cellulose) only while the rats of group III, IV and V were treated with Amlodipine Besylate at the dose 2.5 mg/Kg, 5 mg/Kg and 10 mg/Kg. A full thickness excision wound of 2 cm diameter was created on the dorsal surface of all rats for measuring the wound area and percent wound contraction on day 0, 2, 5, 9, 12 and 15. Haematological, coagulation and various other biochemical parameters were also assessed on day 15. Changes in the feed consumption and body weight during week 1 and week 2 were recorded. Results: As compared to group I, the mean body weight of rats belonging to group II, III, IV and V was reduced significantly, while the feed consumption during week 2 was significantly enhanced. The rate of wound contraction in group V was better than all other groups. Conclusion: Based on results, it could be concluded that Amlodipine Besylate shows its maximum therapeutic effect at the dose of 10 mg/Kg for diabetic wound healing.

Keywords: Wound healing, Diabetes, Excision, Streptozotocin (STZ).