Abstract

THE BIOLOGICAL EFFECT OF B-SITOSTEROL ON HEPATOMA CELL HEPG2 TRANSFECTED WITH HBX GENE

Ammar M. Lubeiny*, WuYun Li, Jin Zhen, Tang Zhang, M. A. Hassan, Yi Qin, Wei Liu, Xu Lin and Elshaikh A. Elobied

Abstract

Hepatitis B virus (HBV) infection is endemic disease in many areas around the world and is considered as one of the risk factor for hepatocellular carcinoma .The latter is the fifth leading cause of death and rapidly increased and became a global health problem. There is an outstanding and urgent need for the development of effective treatment for chronic hepatitis and HCC. The aim of this study was to impair the hepatitis b virus replication and cancer metastasis. To explore this issue we investigated the effect of β-Sitosterol on biological process of hepatoma cell line. HepG2 transfected with hepatitis B x gene. The cell migration and gene expression were studied using gene chip. Gene Ontology result shows that β-Sitosterol has an effect on HepG2-HBx. The Gene Set Enrichment Analysis shows the impact of β-Sitosterol is negative regulation of megakaryocyte, telomere maintenance and extrinsic apoptotic signaling pathway. This result may open new areas of hepatitis B research.

Keywords: HCC, HBV, gene expression, cell migration megakaryocyte, telomere maintenance.